Fig. 2 | Scientific Reports

Fig. 2

From: A robust high-throughput screening system to assess bacterial tyrosine ammonia lyase activity in the context of tyrosine inherited metabolic disorders

Fig. 2

Methodology of the optimized HTS assay. E. coli BL21 (DE3) pET42b( +)-FjTAL cells were cultured and FjTAL expression was induced followed by incubation under the conditions that yield the highest FjTAL concentration. Afterwards, bacteria were lysed and the bacterial cell lysate, containing the FjTAL enzyme, was used to perform the HTS assay.

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