Fig. 4 | Scientific Reports

Fig. 4

From: A robust high-throughput screening system to assess bacterial tyrosine ammonia lyase activity in the context of tyrosine inherited metabolic disorders

Fig. 4

(a) Influence of pH and temperature on bacterial TAL activity. There was a significant difference between both tested conditions at pH 9.2 as well as between both conditions tested at pH 7.4 (P < 0.0001). Furthermore, assay performance was significantly better at 37 °C and pH 9.2 compared to testing at 25 °C at pH 7.4 (P < 0.0001) and 37 °C at pH 7.4 (P < 0.0001). (b) Michaelis–Menten graphs for each ratio of cell lysate containing FjTAL to assay mix containing various concentrations Tyr. (c) Analysis of the Vmax values for each ratio of cell lysate containing FjTAL to assay mix containing Tyr. Based on (b) and (c), a 20/80 ratio of cell lysate to assay mix containing 1 mM Tyr were chosen as ideal assay conditions as here the highest Vmax was obtained while the FjTAL enzyme was saturated.

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