Table 2 Data of inter-plate and inter-day variation. All inter-plate and inter-day fold shifts should be < 2.

From: A robust high-throughput screening system to assess bacterial tyrosine ammonia lyase activity in the context of tyrosine inherited metabolic disorders

 

Plate 1–Plate 2

Plate 1–Plate 3

Plate 2–Plate 3

Inter-plate

Day 1

1.2

1.3

1.1

Day 2

1.0

1.1

1.1

Day 3

1.1

1.1

1.0

Inter-day

Day 1–Day 2

1.1

Day 1–Day 3

1.1

Day 2–Day 3

1.0

  1. As the criteria of both the plate uniformity study and the inter-plate and inter-day variations were fulfilled, the assay could be regarded as excellent and robust under the optimized assay conditions.
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