Fig. 2 | Scientific Reports

Fig. 2

From: Exosomes carrying adipose mesenchymal stem cells function alleviate scleroderma skin fibrosis by inhibiting the TGF-β1/Smad3 axis

Fig. 2

Effects of direct contact of AMSCs on SSc fibroblasts. (A) Schematic diagram of direct contact co-culture; (B–D) Real-time PCR was performed to detect α-SMA, COL3A1, and TGF-β1 mRNA in direct co-culture of AMSCs and SSc skin fibroblasts for 48 h. (E–H) Western blot analysis of the α-SMA, COL3A1,TGF-β1 proteins in direct co-culture of AMSCs and SSc skin fibroblasts for 48 h. Data are presented as the mean ± SD, *p < 0.05, **p < 0.01.

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