Fig. 3 | Scientific Reports

Fig. 3

From: Exosomes carrying adipose mesenchymal stem cells function alleviate scleroderma skin fibrosis by inhibiting the TGF-β1/Smad3 axis

Fig. 3

Effects of indirect contact of AMSCs on SSc fibroblasts. (A) Schematic diagram of indirect contact co-culture; (B–E) Real-time PCR was performed to detect α-SMA, COL1A1, COL3A1, TGF-β1 mRNA in indirect co-culture of AMSCs and SSc fibroblasts for 48 h. (F–J) Western blot analysis of the α-SMA, COL1A1, COL3A1,TGF-β1 proteins in indirect co-culture of AMSCs and SSc fibroblasts for 48 h. Data are presented as the mean ± SD, *p < 0.05, **p < 0.01, and ***p < 0.005.

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