Fig. 7 | Scientific Reports

Fig. 7

From: High mobility group box-1 protein promotes astrocytic CCL5 production through the MAPK/NF-κB pathway following spinal cord injury

Fig. 7

Effects of HMGB1-induced astrocytic CCL5 on microglia/macrophage polarization. (A) and (B) qRT-PCR was used to determine the expression of M1 markers (CD86, iNOS and TNF-a) and M2 markers (CD206, Arg1 and Ym1) after BV2 or RAW 264.7 cells incubated with 0.1 µg/ml rCCL5 for 24 h. (C) and (D) The expression levels of M1 and M2 markers were determined by qRT-PCR following BV2 or RAW 264.7 cells cocultured with ACM for 24 h. ACM was prepared by astrocytes challenge with 0.5 µg/ml rHMGB1 for 24 h, after which the supernatant was collected and incubated with 2.5 µg/ml IgG or CCL5 Ab for 4 h. The experiments were performed in triplicate. The error bars represent the standard deviation (*P < 0.05, **P < 0.01, ***P < 0.001).

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