Fig. 3 | Scientific Reports

Fig. 3

From: Implications of ITCH-mediated ubiquitination of SIX1 on CDC27-cyclinB1 signaling in nasopharyngeal carcinoma

Fig. 3

ITCH was involved in ubiquitination degradation of SIX1 in NPC cell lines. (A) Molecular docking analysis of the interaction between ITCH and SIX1. (B) Immunofluorescence showed colocalization of ITCH (green) and SIX1 (Red) in 6-10B cells. (C,D) The western blot demonstrated the effect of gradient overexpression of ITCH on SIX1 protein expression in 6-10B cells. The statistical chart was displayed below it. (E,F) COIP assays showed the interaction between SIX1 and ITCH in 293T and 6-10B cells. G The western blot demonstrated the efficiency of ITCH overexpression in 6-10B cells. (H) Immunofluorescence showed the effect of ITCH overexpression on SIX1 colocalization in 6-10B cells. (I,J) COIP experiments confirmed that the E3 ubiquitination ligase ITCH promoted the ubiquitination level of SIX1. *P < 0.05.

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