Fig. 6

SIX1 knockdown inhibited 6-10B tumorigenicity in vivo. Four-week-old nude mice (six mice in each group) were injected subcutaneously with knockdown SIX 6-10B cells and control 6-10B cells (5 × 106 cells per mouse) and tumor size was measured starting on day 7. (A) Weight of nude mice, (B) photographs of 6-10B tumor formation in the xenografts of nude mice. (C–E) Picture of isolated tumors, tumor weight, and tumor volume. (F) The typical images -stained by H&E were presented, (G,H) Ki67 and SIX1 IHC staining, The statistical analysis was displayed on the side. The data are presented as the mean ± SD values. *P < 0.05; **P < 0.01; ***P < 0.001. shNC-SIX1: negative control; sh-SIX1:SIX1 knockdown.