Fig. 2

Phylogenetic tree of Toxoplasma gondii based on the partial ITS-1gene sequences. The four new sequences of Toxoplasma gondii obtained in this study are highlighted in red (OR797081-OR797084). Scale bar represents 0.02 substitutions per nucleotide position. Bootstrap value is shown as number on the node. The evolutionary history was inferred by using the Maximum Likelihood method and Kimura 2-parameter model⁵². The tree with the highest log likelihood (− 511.25) is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches. Initial tree(s) for the heuristic search were obtained automatically by applying Neighbor-Join and BioNJ algorithms to a matrix of pairwise distances estimated using the Maximum Composite Likelihood (MCL) approach, and then selecting the topology with superior log likelihood value. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The proportion of sites where at least 1 unambiguous base is present in at least 1 sequence for each descendent clade is shown next to each internal node in the tree. This analysis involved 26 nucleotide sequences. There was a total of 242 positions in the final dataset. Evolutionary analyses were conducted in MEGA X ⁵³.