Fig. 2
METTL3 regulates the proliferation, migration, invasion and apoptosis of CC cells in vitro. (A) The expression of METTL3 in CC cells transfected with normal control (siNC), METTL3-targeted (siMETTL3-1#, siMETTL3-2#, and siMETTL3-3#) siRNAs was detected by qRT-PCR. (B) The mRNA expression level of METTL3 in METTL3 up- or down-regulated CC cells was confirmed by qRT-PCR. (C) The protein expression level of METTL3 in METTL3 up- or down-regulated CC cells was confirmed by western blot analysis. (D) The effect of METTL3 on CC cells proliferation at different time points was measured by CCK-8 assay. (E) The effect of METTL3 on CC cells migration was detected by wound healing assay. (F) The effect of METTL3 on CC cells invasion was tested by transwell assay. (G) The effect of METTL3 on CC cells apoptosis was detected by flow cytometry. (H) qRT-PCR indicated the Twist, Snail, and E-cadherin expression in the transfection of METTL3 overexpression. *P < 0.05. **P < 0.01. ***P < 0.001.
