Fig. 5

Inflammatory priming provokes the expression of high levels of several chemokines and IDO by hMuSCs. (A-F) Expression levels of chemokines and IDO in hMuSCs stimulated with human IFN-γ and TNF-α (10 ng/ml each) for 24 h were assayed by qRT-PCR (n = 3). (G) The protein expression of IDO and β-ACTIN (loading control) were determined by immunoblotting in hMuSCs stimulated with human IFN-γ and TNF-α (10 ng/ml each) for 24 h (n = 3). The original images of blots are in the Supplementary Information. Values are presented as mean ± SEM. Statistical analysis was performed by two-tailed unpaired t test.