Fig. 1
Silencing FEZF1-AS1 abrogates TGF-β1-induced HLF and A549 cell proliferation, migration and invasion in vitro. (A) Genomic locus of LncRNA FEZF1-AS1. (B) qRT-PCR analysis of the expression of FEZF1-AS1 in cultured HLF and A549 treated with TGF-β1. (C) The efficiency of FEZF1-AS1 knockout vector transfection was assessed via qPCR. (D) qPCR analysis of FEZF1-AS1 after treated with TGF-β1 with or without FEZF1-AS1 knockdown. (E) CCK8 assay was used to test the effect of FEZF1-AS1 knockdown on TGF-β1-treated HLF and A549 proliferation. (F) Colony formation assay (seeded at 6-well plate) indicated the effect of FEZF1-AS1 inhibition on TGF-β1-induced HLF and A549 proliferation. (G) Wound-healing assay was used to evaluate the migration of TGF-β1-treated HLF and A549 after FEZF1-AS1 depletion. (H) Representative images of transwell invasion assay (left) and quantification of cell invasion ability (right) for FEZF1-AS1’s role in TGF-β1-treated HLF and A549 cells. Control, HLF and A549 cell; sh-NC, TGF-β1-treated HLF and A549 cells transfected with a non-targeting shRNA sequence (sh-NC), serving as a control for FEZF1-AS1 knockdown; sh-1, TGF-β1-treated HLF and A549 cell with FEZF1-AS1 knockdown (D-H). All data were presented as mean ± SD. n = 3 independent experiments. *p < 0.05.
