Fig. 2 | Scientific Reports

Fig. 2

From: LncRNA FEZF1-AS1 promotes pulmonary fibrosis via up-regulating EZH2 and targeting miR-200c-3p to regulate the ZEB1 pathway

Fig. 2

Silencing FEZF1-AS1 mitigated fibrosis-related proteins changes induced by TGF-β1 and the localization of FEZF1-AS1. (A) Western blot analysis of EMT-related proteins in TGF-β1-treated A549. (B) Western blot analysis of the proteins associated with fibroblast-myofibroblast transition in TGF-β1-treated HLF. (C) Western blot analysis of EMT-related proteins in TGF-β1-treated A549 cells after FEZF1-AS1 silencing. (D) Western blot analysis of the proteins associated with fibroblast-myofibroblast transition in TGF-β1-treated HLF after FEZF1-AS1 silencing. (E) The fluorescent microscopic images of A549 and HLF cells show the distribution of the FAM dye in the cytoplasm and nucleus. Yellow arrows indicate nuclear regions, while green arrows indicate the cytoplasmic region. Fluorescent signals are expressed in both regions, indicating that FEZF1-AS1 is localized in both cytoplasm and nucleus. All data were presented as mean ± SD. n = 3 independent experiments. *p < 0.05.

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