Fig. 4 | Scientific Reports

Fig. 4

From: LncRNA FEZF1-AS1 promotes pulmonary fibrosis via up-regulating EZH2 and targeting miR-200c-3p to regulate the ZEB1 pathway

Fig. 4

FEZF1-AS1 regulates the expression and activity of miR-200c-3p by acting as a ceRNA. (A) qRT-PCR analysis of miR-200c-3p expression in TGF-β1 treated A549 and HLF cells (10 ng/ml) for 48 h. (B) qRT-PCR analysis of miR-200c-3p expression after FEZF1-AS1 inhibition in TGF-β1-treated HLF and A549. (C) Sequence alignment of miR-200c-3p with the predicted binding sites within the wild-type or mutated FEZF1-AS1 regions. (D) Luciferase reporter activity of vectors carrying the luciferase gene and a fragment of FEZF1-AS1 containing wild-type or mutated miR-200c-3p binding site. All data were presented as mean ± SD. n = 3 independent experiments. *p < 0.05.

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