Table 1 Comparative physiological characteristics of two novel strains, DS1-an-13321T and DS1-an-2312T, and their representative reference strains in the family Prolixibacteraceae. Strains: 1, DS1-an-13321T; 2, DS1-an-2312T; 3, Puteibacter caeruleilacunae JC036T43; 4, Prolixibacter bellariivorans JCM 13498T75; 5, Sunxiuqinia elliptica CGMCC 1.9156T77; 6, Maribellus luteus XSD2T22; 7, Draconibacterium orientale FH5T40; 8, Aquipluma nitroreducens MeG22T37; 9, Mangrovibacterium diazotrophicum DSM 27148T39. 10, Mariniphaga anaerophila DSM 26910T42; 11, Tangfeifania diversioriginum DSM 27063T81; 12, Gaoshiqia Sediminis A06T76. * data obtained from43.
Characteristic | 1 | 2 | 3 | 4 | 5 | 6 |
|---|---|---|---|---|---|---|
Colony | Nearly round shape colony and bright brown color | Irregular shape, bright brown color | Creamy white* | White* | Slightly pink* | Light orange |
Cell diameter (µm) (width × length) | Long-rod shape, long-shape: 0.25–0.5 × ~20, Spherical shape: 0.48–0.95 | Long-rod shape, long-shape: 0.25–0.5 × ~20, Spherical shape: 0.48–0.95 | Rod shape, 0.3–0.5 × 1–3 | Filamentous-rod shape, 0.33 × 10.5–12.5 | Rod shape, 0.4 × 0.8–0.9 | 0.6–1.0 × 2.0–9.0 |
Temperature range (℃) | 15–30 | 10–32 | 4–37 | 4–42 | 15–42 | 20–40 |
Optimum growth temperature (℃) | 20–30 | 20–30 | 28 | 22 | 30 | 33 |
pH range (optimum) | 6.0–8.5 (6.5–8.5) | 6.0–8.0 (7.0–7.5) | 5.0–9.0 (7.0) | 5.0–9.0 (7.0) | 5.0–9.0 (7.0–8.0) | 6.0–8.5 (7.0–7.5) |
NaCl (%) tolerance (optimum) | 2–4 (2–3) | 1–4 (2–3) | 2–6 (3) | 0.5–8 (2) | 0.5–10 (3) | 1–5 (3) |
Oxygen requirement | Strictly anaerobic | Microaerophilic | Facultative anaerobic | Facultative anaerobes | Strictly aerobic | Facultative anaerobic |
H2S production | + | + | + | − | + | + |
Hydrolysis | ||||||
Gelatin | + | + | + | − | − | + |
Starch | + | + | − | + | − | + |
DNase | + | + | – | − | ND | − |
Utilization | ||||||
Fructose | − | − | + | − | + | ND |
Galactose | + | + | + | + | − | ND |
Lactose | + | + | ND | + | − | ND |
Sucrose | + | + | + | + | ND | ND |
Xylose | − | + | ND | + | + | + |
Source of isolation | Sea squirt at a depth of 18 m | Sea squirt at a depth of 18 m | Seawater at a depth of 90 m at Yongle blue Hole in the South China sea. | Surface of an electricity-harvesting electrode incubated in marine sediments. | Sediment of a seashore pond for sea cucumber culture in Jimo, Qingdao, on the east coast of China. | Coastal seawater of Xiaoshi Island, Weihai, PR China |
Characteristic | 7 | 8 | 9 | 10 | 11 | 12 |
|---|---|---|---|---|---|---|
Colony | Light pink | Beige | Pink and change to orange–red | ND | Pink | Orange |
Cell diameter (µm) (width × length) | 0.3–0.5 × 1.3–1.8 | 0.8 × 2.5–10 | 0.45–0.55 × 5.5–6.5 | 0.5–0.6 × 1.9–6.9 | 0.25–0.3 × 2.3–4.3 | 0.4–0.5 × 0.6–1.0 |
Temperature range (℃) | 20–40 | 4–32 | 25–30 | 20–40 | 25–40 | 20–40 |
Optimum growth temperature (℃) | 28–32 | 30 | ND | 35–37 | 33–35 | 33 |
pH range (optimum) | 5.5–9.0 (7.0–7.5) | 4.2–8.9 | 6.0–8.0 | 6.0–8.5 | 6.5–8.5 | 6.0–8.0 |
NaCl (%) tolerance (optimum) | 2–4 | 0–0.25 (0) | 0–2 (0.5) | 0–8 (1–2) | 1–8 (2–4) | 0–8 (2) |
Oxygen requirement | Facultative anaerobic | Facultative anaerobic | Facultative anaerobic | Facultative anaerobic | Facultative anaerobic | Facultative anaerobic |
H2S production | – | ND | – | ND | – | ND |
Hydrolysis | ||||||
Gelatin | + | ND | + | ND | + | ND |
Starch | – | ND | – | + | + | + |
DNase | ND | ND | ND | ND | ND | – |
Utilization | ||||||
Fructose | + | + | – | – | – | + |
Galactose | + | + | – | ND | + | + |
Lactose | – | – | – | + | + | + |
Sucrose | + | + | – | + | ND | ND |
Xylose | + | + | + | + | + | ND |
Source of isolation | Sediment sample, from the coastal area of Weihai, China | Freshwater lake in Japan | Mangrove sediment at Bailu Park, Sanya city, on the south coast of China. | Tidal-flat sediment from Tokyo Bay, Chiba, Japan | Saltwater lake located in Qinghai Province, China | Marine sediment sample, from the coast of Weihai, PR China |
