Figure 7
Plaque-forming efficiencies of representative strains harboring different BimABptypes in A549 cells. (a) Photographic representation of plaques. (b) Plaque-forming efficiencies of B. pseudomallei isolates harboring the different BimABp types in A549 cells. The cells were infected with B. pseudomallei strains representative of BimABp type 1 (B. pseudomallei K96243, classified as type 1 was used as the reference strain), type 2 (DR10025A, DR20021A and DR40130A), type 4 (DR40111A, DR80025A and DR90085A), type 6 (DR10008A, DR40025A and DR50053A), type 9 (DR50173A, DR70003A and DR90006A), and type 10 (DR20062A, DR50003A and DR60054A) at MOI of 0.1:1. Plaques were stained with 2% (w/v) crystal violet at 24 h post-infection. Plaque-forming efficiency (PFU/ml) was counted as the number of plaques (plaque-forming units: PFU) divided by the CFU (colony-forming units) of bacteria added per well (CFU/ml). Error bars represent means ± standard deviation of data from three independent experiments (one-way ANOVA; P < 0.05).
