Figure 8
Confocal microscopy images of A549 cells infected with B. pseudomallei isolates with different BimABptypes. The representative strains of B. pseudomallei (a) BimABp type 1 (B. pseudomallei K96243, classified as type 1 was used as the reference strain), (b) BimABp type 2 (DR40130A), (c) BimABp type 4 (DR40111A), (d) BimABp type 6 (DR10008A), (e) BimABp type 9 (DR50173A), and (f) BimABp type 10 (DR50003A) were used to infect A549 cells (g) at MOI of 30:1. Immunofluorescence staining was performed at 8 h post-infection using 4B11 monoclonal antibody specific to B. pseudomallei capsular polysaccharide to visualize the bacteria in green; phalloidin for F-actin in red; and Hoechst 33258 for the host DNA in blue. (h) The length of actin tails (white arrow) was determined using Zen Zeiss 3.0 SR (black) software tools. The mean lengths were: 3.1605 μm, BimABp type 1; 1.988 μm, BimABp type 2; 2.043 μm, BimABp type 4; 1.807 μm, BimABp type 6; 1.465 μm, BimABp type 9; and 1.603 μm, BimABp type 10 (one-way ANOVA; P < 0.05). Scale bar, 10 μm.
