Fig. 1 | Scientific Reports

Fig. 1

From: SLC37A4, gene responsible for glycogen storage disease type 1b, regulates gingival epithelial barrier function via JAM1 expression

Fig. 1

SLC37A4 localization in human gingival epithelial cells. (a) RT-PCR analysis of SLC37A4 gene in IHGE cells. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the control. (b) IHGE cells expressing Myc-SLC37A4 and EGFP-SEC61β (green) were fixed, stained with anti-Myc (magenta: Alexa Fluor 555), and analyzed by confocal microscopy. The area enclosed by the white square area showing the periodontal pocket in the upper panel is magnified in the lower panel. (c-e) IHGE cells expressing Myc-SLC37A4 were fixed, stained with anti-Myc (green: FITC) and either anti-TOMM20 (magenta: Alexa Fluor 555 in c), anti-TGN46 (magenta: Alexa Fluor 555 in d), or anti-LAMP1 (magenta: Alexa Fluor 555 in e), then analyzed by confocal microscopy. The white square areas showing the periodontal pocket in the left panels are magnified in the right panels. Scale bars, 10 μm. See also Supplementary Fig. 1.

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