Fig. 2 | Scientific Reports

Fig. 2

From: SLC37A4, gene responsible for glycogen storage disease type 1b, regulates gingival epithelial barrier function via JAM1 expression

Fig. 2

Effects of SLC37A4 knockout on phenotype of IHGE cells. (a) IHGE WT or ΔSLC37A4 cells were fixed, stained with DAPI (cyan) and rabbit polyclonal anti-SLC37A4 (gray: Alexa Fluor 555, Proteintech 20,612–1-AP), and analyzed by confocal microscopy. (b-d) IHGE WT or ΔSLC37A4 cells were fixed, stained with DAPI (cyan) and either concanavalin A (gray: Alexa Fluor 594), rabbit polyclonal anti-TOMM20 (gray: Alexa Fluor 555 in c), or mouse monoclonal anti-JAM1 (gray: Alexa Fluor in d), then analyzed by confocal microscopy. The white square areas in the upper panels of b and c are magnified in the lower panels. Arrows in knockout cells indicate a pattern different from WT cells. Scale bars, 5 μm.

Back to article page