Fig. 3 | Scientific Reports

Fig. 3

From: SLC37A4, gene responsible for glycogen storage disease type 1b, regulates gingival epithelial barrier function via JAM1 expression

Fig. 3

HMX3 involved in SLC37A4 knockout-reduced JAM1 expression in IHGE cells. (a) NFIA, HMX3, GATA1, and PAX2 mRNA expression in IHGE cells, with results expressed as fold change relative to NFIA and shown as the mean of five technical replicates (bars). ND, not detected after 45 cycles of qRT-PCR. (b, d) HMX3 (b) and JAM1 (d) mRNA expression in IHGE WT and SLC37A4 knockout cells with HA-HMX transient transfection, with results expressed as fold change relative to WT cells and shown as the mean of five technical replicates (bars). Significant differences were determined using a two-tailed t test (closed-testing procedure). (c) NFIA mRNA expression in IHGE WT and SLC37A4 knockout cells, with results expressed as fold change relative to WT cells and shown as the mean of five technical replicates (bars). Significant differences were determined using a two-tailed t test. (e, f) HMX3 (e) and SLC37A4 (f) mRNA expression in IHGE cells with HA-HMX transient transfection, with results expressed as fold change relative to no transfection (Control) and shown as the mean of five technical replicates (bars). Significant differences were determined using a two-tailed t test. *p < 0.05. β-ACTIN was used as the internal control. The results shown are representative of two biological replicates. Dot plots display data values for each experimental condition.

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