Fig. 4 | Scientific Reports

Fig. 4

From: SLC37A4, gene responsible for glycogen storage disease type 1b, regulates gingival epithelial barrier function via JAM1 expression

Fig. 4

HMX3 knockdown results in decreased JAM1 expression in IHGE cells. (a) RT-PCR analysis of HMX3 gene in IHGE cells. β-ACTIN was used as the control. (b, c) HMX3 (b) and JAM1 (c) mRNA expression in IHGE cells stably expressing shLuc or shHMX3 (#644 or #737) is shown as fold change relative to shLuc-expressing cells, and presented as the mean of five technical replicates (bars). Significant differences were determined using Dunnett’s test. *p < 0.05. The results shown are representative of two biological replicates. (d) Lysates of IHGE cells expressing indicated shRNA were analyzed by immunoblotting with the indicated antibodies. (e) IHGE cells stably expressing shLuc or shHMX3 (#644 or #737) were fixed, then stained with DAPI (cyan) and mouse monoclonal anti-JAM1 (gray: Alexa Fluor 555), then analyzed by confocal microscopy. Scale bars, 10 μm.

Back to article page