Fig. 5 | Scientific Reports

Fig. 5

From: SLC37A4, gene responsible for glycogen storage disease type 1b, regulates gingival epithelial barrier function via JAM1 expression

Fig. 5

JAM1 involved in SLC37A4 knockout-disturbed barrier against LPS and PGN in IHGE cells. (a) Schematic image of culture-insert system. A monolayer of IHGE WT or SLC37A4 knockout cells along with stable expression of HA-inserted JAM1 was cultured in the upper compartment. Fluorescent tracers were added and culturing was performed for 30 min, after which culture medium from the lower compartment was analyzed using spectrometry. (b) Representative confocal microscopic images of IHGE WT or SLC37A4 knockout cells along with stable expression of HA-inserted JAM1. DAPI (cyan), anti-JAM1 (magenta: Alexa Fluor 555), anti-HA (gray: Alexa Fluor 647). (c, d) Permeability to FITC-P. gingivalis LPS (c) or FITC-S. aureus PGN (d) in indicated IHGE cells. Results are expressed as fold change relative to WT cells and presented as the mean ± SD of eight technical replicates. *p < 0.05, two-tailed t test (closed-testing procedure). The results shown are representative of two biological replicates.

Back to article page