Fig. 2
Identification of DEGs and WGCNA key modules in CKD. (A,B) Gene expression density map before (A) and after (B) batch correction of CKD datasets. (C,D) PCA plots before (C) and after (D) batch correction of CKD datasets. (E) Volcano plot illustrating the DEGs in the CKD combined dataset. In the visual representation, genes that are upregulated are symbolized by red dots, whereas genes that are downregulated are symbolized by blue dots. (F) Heatmap featuring the top 30 upregulated and top 30 downregulated DEGs in the CKD combined dataset. (G) Gene dendrogram displaying the cleaved gene modules. (H) Heatmap highlighting the associations of MEs with CKD status, with blue and pink modules exhibiting significant correlations with CKD (p < 0.05). (I) Venn diagram illustrating the identification of 225 CKD key genes through the intersection of key module with DEGs. DEG, differentially expressed gene; WGCNA, weighted gene co-expression network analysis; CKD, chronic kidney disease; ME, module eigengene.
