Fig. 4

Expression of RNF144B in tumors from patients with HGSOC. (a) Representative IHC staining of RNF144B in ovarian surface epithelium (OSE), fallopian tube (FT), and HGSOC tumors (n = 100) using anti-RNF144B antibody at concentration 1:100. (b) RNF144B protein expression levels in control and HGSOC tissues, p = 0.03. (c) RNF144B immunoreactivity in a panel of matched pair samples (n = 50). Tissues were collected from the same patient at the time of diagnostic biopsy and during surgery following 3–4 cycles of chemotherapy. (d) Plot showing the distribution of RNF144B intensity scores in matched pair samples, analyzed using two-tailed Student’s t-test, p < 0.0001. (e) Expression of RNF144B in patients who recurred (n = 33) compared to those in complete remission (n = 17). The bar graph represents the mean value of the difference in IHC scores between pre- and post-treated tumors obtained from patients who had either recurrence or complete remission, p = 0.821. The P-value was calculated using the Wilcoxon signed rank test. Magnification 40x (Microscope Nikon E200 Japan).