Table 3 Application of recombinase polymerase amplification (RPA) in the detection isolaters of D. graminicola in infected plant tissues.
From: Establishment of recombinase polymerase amplification detection method for Dactylobotrys graminicola
Location | Samples | Sequence | PCR assay | RPA assay |
|---|---|---|---|---|
23-DGC | Healthy flag leaf | 1 | − | − |
Healthy flag leaf | 2 | − | − | |
Disease stems | 3 | + | + | |
Disease stems | 4 | + | + | |
Healthy spikes | 5 | − | − | |
Healthy spikes | 6 | − | − | |
23-YGC | Disease flag leaf | 7 | + | + |
Healthy flag leaf | 8 | − | − | |
Healthy stems | 9 | − | − | |
Healthy stems | 10 | − | − | |
Healthy spikes | 11 | − | − | |
Disease spikes | 12 | + | + | |
23-DTC | Disease flag leaf | 13 | + | + |
Disease flag leaf | 14 | + | + | |
Healthy stems | 15 | − | − | |
Disease stems | 16 | + | + | |
Healthy spikes | 17 | − | − | |
Healthy spikes | 18 | − | − | |
23-GMNXC | Healthy flag leaf | 19 | − | − |
Healthy flag leaf | 20 | − | − | |
Disease stems | 21 | + | + | |
Disease stems | 22 | + | + | |
Disease spikes | 23 | + | + | |
Disease spikes | 24 | + | + | |
23-SJWC | Disease flag leaf | 25 | + | + |
Disease flag leaf | 26 | + | + | |
Healthy stems | 27 | − | − | |
Disease stems | 28 | + | + | |
Disease spikes | 29 | + | + | |
Healthy spikes | 30 | − | − | |
23-BMZ | Disease flag leaf | 31 | + | + |
Disease flag leaf | 32 | + | + | |
Healthy stems | 33 | − | − | |
Healthy stems | 34 | − | − | |
Disease spikes | 35 | + | + | |
Disease spikes | 36 | + | + |
