Fig. 4 | Scientific Reports

Fig. 4

From: ASK-1 activation exacerbates kidney dysfunction via increment of glomerular permeability and accelerates cellular aging in diabetic kidney disease model mice

Fig. 4

ASK-1 activation induce tubular damage and interstitial fibrosis in DKD. (A) Masson trichrome staining (bar = 100 μm). (B) Expression of fibrogenic gene (alpha smooth muscle actin [α-SMA], Collagen type I [Col I], Fibronectin 1 [FN 1] and Transforming growth factor-β [TGF-β]) were assessed with quantitative reverse transcription PCR (qRT-PCR). (C) Immunohistochemical staining for KIM-1 (bar = 100μm). (D) ELISA quantifying kidney expression of KIM-1. Data are expressed as means ± SEM. *P < 0.05 versus db/misty; †P < 0.05 versus eNOS-KO db/db. db/misty control; db/misty ASK-1 inhibitor; eNOS-KO db/db control; eNOS-KO db/db ASK-1 inhibitor. The number of mice n = 6–11.

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