Fig. 6 | Scientific Reports

Fig. 6

From: ASK-1 activation exacerbates kidney dysfunction via increment of glomerular permeability and accelerates cellular aging in diabetic kidney disease model mice

Fig. 6

Redox status induced cellular senescence via ASK-1 activation in tubular cells. (A) Representative images of SA–β-gal–stained hPTECs. (B) Immunoblot analysis quantifying cell expression of p21 and GAPDH. Original blots are presented in Supplementary Fig. 10-A,B. (C) p21 protein level expressed as fold change. Data are expressed as means ± SEM. *P < 0.05 versus control. (D) In vivo, representative western blotting experiments for p53, p21 and GAPDH. These marker protein level expressed as fold change in Supplementary Fig. 7. Original blots are presented in Supplementary Fig. 10-C. (E) Expression of SASP related gene (interleukin-1β[IL-1β], interleukin-18[IL-18], F4/80) were assessed with quantitative reverse transcription PCR (qRT-PCR). Data are expressed as means ± SEM. *P < 0.05 versus eNOS-KO db/db control.

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