Table 2 The primer and probe sequences for qRT-PCR.
Gene | ID | NAME | Sequence |
|---|---|---|---|
aSMA | NM_007392 | Forward primer | caggcattgctgacaggat |
Reverse primer | gttctggaggggcaatgat | ||
Probe | ctcgcacccagcaccatgaaga | ||
COL1a1 | NM_007742 | Forward primer | tgtgcgatgacgtgcaat |
Reverse primer | ttgggtccctcgactcctac | ||
Probe | actggactgtcccaacccccaaag | ||
FN1 | NM_010233 | Forward primer | atgatgaggtgcacgtgtgt |
Reverse primer | tgacgcttgtggaacgtgt | ||
Probe | tcgtggagaatgggcatgca | ||
TGFβ | NM_011577 | Forward primer | acctgcaagaccatcgacatg |
Reverse primer | cgagccttagtttggacaggat | ||
Probe | aagcgcatcgaagccatccgtg | ||
GAPDH | NM_008084 | Forward primer | aatgaccccttcattgacctc |
Reverse primer | gtcttctgggtggcagtgat |
