Fig. 3
From: Minicircle-based vaccine induces potent T-cell and antibody responses against hepatitis C virus
Analysis of cellular response in BALB/c mice immunized with MC. Box plot of antigen-specific IFN-γ ELISPOT responses in MC groups. Splenocytes from six immunized BALB/c mice per group were stimulated with overlapping peptides spanning the sequences of sHBsAg, NS3, NS4 and NS5. Additionally for the mice immunized with sHBsAg_412–425 MC, sHBsAg peptides were divided into three groups (P1, P2, P3), corresponding to sHBsAg amino acid 1–99 (P1), 100–188 (P2) and 189–240 (P3). The splenocytes were collected 4 weeks after the last immunization. The box marks 25–75th percentile; the horizontal line indicates the median; whiskers extend to minimal and maximal values. Data was analyzed using two-way analysis of variance (ANOVA) (ns, P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001).
