Fig. 6
From: Minicircle-based vaccine induces potent T-cell and antibody responses against hepatitis C virus
Reactivity of immune sera to a panel of E1E2 complex mutants in which residues across the 412–425 epitope were individually replaced by alanine. E1E2 heterodimer mutants were expressed in HEK293 cells, captured using GNA-coated ELISA plates and probed with immune sera (diluted 1:500) or AP33 mAb (diluted 1:2,500). The mean A450 values are shown on axis y. The substitution introduced to the E1E2 complex are annotated on axis x according to the amino acid occurring within the H77C sequence, and the amino acid position is relative to the H77C polyprotein chain. The data represent the results from two independent experiments performed in duplicate, and error bars indicate standard deviations.
