Fig. 6
From: Vitamin D receptor and its antiproliferative effect in human pulmonary arterial hypertension
The antiproliferative effect of calcitriol is suppressed in the presence of survivin inhibition. (A) BIRC5 (gene encoding survivin) mRNA expression assessed by qRT-PCR 48 h post-transfection with siRNA against BIRC5 (siBIRC5) or scramble siRNA. Data is expressed as scatter plots and bars represent the median, *p < 0.05, Mann–Whitney test. (B, C) Proliferation of PASMC transfected with siBIRC5 or scramble (B) or YM155 (20 nmol/l) measured by MTT and BrdU assays. (D, E) Effects of calcitriol (1–100 nmol/l) on proliferation in PASMC transfected with siBIRC5 or scramble (D) or treated with YM155 (20 nmol/l) (E) measured by BrdU assay for 48 h. Data are expressed as mean ± SEM. *p < 0.05 and **p < 0.01 vs scramble or calcitriol, two-way ANOVA, Bonferroni test. n = 3–4 different cultures in triplicate.
