Fig. 1
From: VP28 interacts with PmRab7 irrespective of its nucleotide state
PmRab7 mutants in this study were designed based on a multiple sequence alignment of PmRab7 against its homologs. Multiple sequence alignment of PmRab7 (DQ231062, Genbank), Rat_Rab7a (NP_076440.1, Genbank), human Rab7 (P51149, Uniprot), human Rab11a (P62491, Uniprot), human Rab11b (Q15907, Uniprot), human KRas; (P01116, Uniprot), human HRas (P01112, Uniprot), and human RhoA (P61586, Uniprot) was constructed using ClustalW program. The G1 to G5 domains of small GTPase proteins are boxes. A GTP/Mg2+ binding site is shown in grey. The switch II region is shown in blue. The star (*), single (.) and double (:) dots represent identical, conserved, and semi-conserved residues in the respective regions, respectively. Arrows indicate each mutated residue in T22N, Q67L, and L129F mutants. The positions of tryptophan residues are highlighted on a dark background.
