Fig. 8
From: VP28 interacts with PmRab7 irrespective of its nucleotide state
The titration of VP28 to PmRab7-WT in the presence of 0.5 mM nucleotide using ITC showed the addition of excess nucleotide (GDP or GTP) enhances the binding affinity. Isothermal titration calorimetry (ITC) titration curve and its corresponding fitting curve of 200 µM of VP28 to 20 µM of PmRab7-WT (a), PmRab7-WT + GDP (b), PmRab7-WT + GTP (c) in 50 mM Tris-HCl pH 8.0, 100 mM NaCl and 5% glycerol with 0.5 mM nucleotide at 25 ºC.
