Table 1 The means of maximum emission wavelength (λmax) of PmRab7 and the percentage of fluorescence emission intensity.

From: VP28 interacts with PmRab7 irrespective of its nucleotide state

 

Nucleotide-free

GDP

GppNHp

λmax, nm

% Fluorescence

at λmax

λmax, nm

% Fluorescence at λmax

λmax, nm

% Fluorescence at λmax

WT

342 ± 3

100

342 ± 0

115

338 ± 1

91

T22N

339 ± 3

100

340 ± 2

117

337 ± 2

122

Q67L

340 ± 1

100

343 ± 2

104

337 ± 1

88

L129F

340 ± 1

100

341 ± 2

113

340 ± 2

102

  1. Note: The percentage of fluorescence emission intensity at the λmax of each mutant was normalized against that of the nucleotide-free form.