Table 3 Thermodynamic parameters of VP28 binding to PmRab7-WT in the presence of excess nucleotides.
From: VP28 interacts with PmRab7 irrespective of its nucleotide state
PmRab7 conformation | KD (nM) | N VP28:Rab7 | ΔH (kcal/mol) | ΔG (kcal/mol) | -TΔS (kcal/mol) | T (ºC) |
|---|---|---|---|---|---|---|
WT (no excess) | 1920 ± 420 | 0.703 ± 2.0e-2 | -20.2 ± 1.35 | -7.80 | 12.4 | 25 |
WT + excess GDP | 924 ± 234 | 0.795 ± 2.0e-2 | -15.4 ± 0.841 | -8.23 | 7.17 | 25 |
WT + excess GTP | 826 ± 198 | 0.738 ± 1.8e-2 | -17.0 ± 0.851 | -8.30 | 8.66 | 25 |
