Table 4 Mutagenic primers for QuikChange in PmRab7.
From: VP28 interacts with PmRab7 irrespective of its nucleotide state
Primer name | Sequences | Purposes |
|---|---|---|
R7_T22N_FWD | GGTGTAGGCAAAAACTCCCTTATGAACCAG | Sense primer for the T22N mutation |
R7_T22N_REV | CTGGTTCATAAGGGAGTTTTTGCCTACACC | Antisense primer for the T22N mutation |
R7_Q67L_FWD | GGGATACAGCTGGTCTCGAGAGATTCCAGT | Sense primer for the Q67L mutation |
R7_Q67L_REV | ACTGGAATCTCTCGAGACCAGCTGTATCCC | Antisense primer for the Q67L mutation |
R7_L129F_FWD | CCTGGGTAACAAGATTGATTTTGAGAATAGGGCGG | Sense primer for the L129F mutation |
R7_L129F_REV | CCGCCCTATTCTCAAAATCAATCTTGTTACCCAGG | Antisense primer for the L129F mutation |
