Table 3 Segregation analysis of S67 alleles in different populations.

From: Identification of a novel hybrid sterility locus S67 between temperate japonica subgroup and basmati subgroup in Oryza sativa L

Population

Generation

Number of plants

Pollen fertility

S67 genotype

χ2 (1:2:1)

χ2 (1:1)

S67

BC4F2

0

186

204

Fully fertility

Semi-sterility

Fully fertility

b/b

b/te

te/te

214.25***

0.83

NIL-S67(H)/DJY1

F1

50

50

Semi-sterility

Fully fertility

b/te

te/te

–

0.00

DJY1/ NIL-S67(H)

F1

0

100

Semi-sterility

Fully fertility

b/te

te/te

–

100.00***

  1. The allele S67-b and S67-te were abbreviated as b and te, respectively, as determined using the SSR marker RM5362. Fourty to sixty percent of pollen fertility was defined as semi-sterility, ~ 90% pollen fertility was defined as fully fertility. Chi-squared tests (χ2) were performed for the segregations of the S67 genotypes in the BC4F2 and F1 and test-cross populations. ***Significant at P < 0.001.
Back to article page