Table 2 Flow cytometry comparison between non-metachronous and metachronous RC.

From: IMMUNOREACT 9 metachronous rectal cancers have high HLA-ABC expression on healthy epithelium but a lower infiltration of CD3+ T cells than primary lesions

 

non-metachronous

Metachronous

p-value

Corrected p-value

CK + CD80 + cell rate

17 (8, 31)

9 (8, 20)

0.514

0.999

CK + CD80 + MFI

129 (101, 174)

182 (150, 229)

0.194

0.221

CK + CD86 + cell rate

16 (7, 28)

25 (20, 30)

0.244

0.325

CK + CD86 + MFI

117 (95, 160)

140 (129, 148)

0.470

0.940

CK + HLAabc + cell rate

35 (14, 67)

40 (24, 52)

0.815

0.999

CK + HLAabc + MFI

412 (239, 602)

905 (763, 976)

0.031

0.033

CD8 + CD28 + cell rate

3.0 (1.0, 6.0)

3.4 (3.3, 6.0)

0.381

0.609

CD8 + CD28 + MFI

42 (30, 64)

33 (29, 38)

0.315

0.458

CD8 + CD38 + cell rate

4 (2, 10)

6 (5, 6)

0.558

0.999

CD8 + CD38 + MFI

49 (37, 66)

49 (44, 56)

0.982

0.982

CD4 + CD25 + cell rate

1.4 (0.0, 3.7)

3.8 (2.3, 4.9)

0.381

0.423

CD4 + CD25 + MFI

19 (0, 34)

24 (14, 36)

0.696

0.999

CD25 + FoxP3 + cell rate

0.00 (0.00, 1.70)

0.00 (0.00, 1.50)

0.797

0.999

CD25 + FoxP3 + MFI

0 (0, 356)

0 (0, 90)

0.554

0.999

CD3 + CTLA4 + cell rate

3 (1, 10)

10 (3, 20)

0.241

0.296

CD3 + CTLA4 + MFI

30 (17, 56)

21 (19, 43)

0.660

0.999

  1. Median (IQR); n (%). Wilcoxon rank sum test. Benjamini and Hochberg False Discovery Rate was used for multiple testing correction.
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