Fig. 2

Representative confocal microscopy images of cells under low energy loading. Under low energy loading without NpPs protection, 74% of cells were viable with intact membranes. Within the viable population, 80.3% exhibited a circular morphology consistent with a healthy status (left) and 19.7% exhibited deformed morphology representing compromised health (right). 26% cells were dead and burst (middle). NpPs addition significantly increased the percentage of healthy cells and decreased the percentages of unhealthy and dead cells under loading.