Fig. 5

Determination of ATP binding site in HomB, HomC, HomP and NisP. ATPbind utilizes the pdb file of protein structure and delivers the potential ATP binding sites with the probability of each residue of respective position. The binding site is considered if the probability value exceeds the threshold of 0.4³⁰. NisP has been employed as a control for the identification of the binding site. HomP possesses several ATP binding sites with a probability value significantly higher than the threshold, unlike HomB and HomC. This suggests that HomP has several possible ATP binding sites that play a role in its energy usage during the proteolytic cleavage of the HomA leader peptide.