Fig. 2
Schematics for the interspecific introgression of mitochondrial DNA. Representative chromosomes are labelled with X (X chromosome), Y (Y chromosome) and A (autosome). Red indicates An. gambiae DNA, blue indicates An. arabiensis DNA, grey indicates hybrid DNA and green denotes the X-linked GFP transgene. (A) Introgression of An. arabiensis mitochondria into an An. gambiae genetic background. WT An. arabiensis females were crossed to An. gambiae X-GFP males in cage. F1 hybrid females were backcrossed to WT An. gambiae males, and transgenic BC1 hybrid females were selected to continue the introgression by repeated backcrosses to WT An. gambiae males. The X-linked GFP transgene is no longer used for the selection of the individuals and is eventually lost. (B) Introgression of An. gambiae mitochondria into an An. arabiensis genetic background. X-GFP An. gambiae females were crossed to WT An. arabiensis males in cage. F1 hybrid females were backcrossed to WT An. arabiensis males and non-transgenic BC1 hybrid females were selected to continue the introgression by repeated backcrosses to WT An. arabiensis males.
