Fig. 3
Genetic crosses performed to assess the effect of An. arabiensis mitochondria in F1 hybrid males containing the An. gambiae X chromosome. Representative chromosomes are labelled with X (X chromosome), Y (Y chromosome) and A (autosome). Red indicates An. gambiae DNA, blue indicates An. arabiensis DNA. (A) GamAm females were crossed in cage to WT An. arabiensis males to generate GamAm/Ara F1 hybrid males containing An. arabiensis mitochondria and An. gambiae X chromosomes. (B) GamAm males were crossed to WT An. gambiae females in cage to generate GamGm females which had the same genomic species-composition as the GamAm strain but contained An. gambiae mitochondria. These females were crossed to WT An. arabiensis males in cage to generate GamGm/Ara F1 hybrid males containing both the mitochondria and the X chromosome from An. gambiae. (C) As a control, F1 hybrid males were generated by crossing WT An. arabiensis males to WT An. gambiae females in cage. The Gam/Ara F1 hybrid males contained a set of autosomes from each species and mitochondria from An. gambiae.
