Fig. 3
High-parameter flow cytometric analysis of stimulated T cells reveals distinct immune profiles in various forms of erythroderma.
(A) Uniform manifold approximation and projection (UMAP) plot of T cell immune populations combined in the index patient, erythroderma controls, and healthy controls. (B) UMAP plot of the expression of each individual marker characterizing functional T cell immune populations. (C) UMAP plot of the differences in T cell immune populations between the index patient, erythrodermic controls, and healthy controls. (D) Bar plots showing percentages of subtypes of CD3 + T cells in the patients. (E) Subpopulations of the percentage of CD3 + T cell types shown in d: γVδ T cells are stratified by γVδ1TCR and γVδ2TCR cells. CD4 T cells are stratified by naive CD4 (CD3 + CD4 + CD45RA+) and memory CD4 (CD3 + CD4 + CD45RO+) T cells. CD8 T cells are stratified by naive CD8 (CD3 + CD8 + CD45RA+) and memory CD8 (CD3 + CD4 + CD45RO+) T cells. (F) FlowSOM metaclustering algorithm demonstrating similarities and differences in the T cell immune populations between each group. Each coded color represents an overlay of the immune cell populations and metaclusters. (G) Bar plots showing the abundance of the metaclusters between the groups.
