Fig. 6
High-parameter flow cytometric analysis reveals modulation of immunopathogenic granulocytic and monocytic immune cell populations with dual inhibition of IL-4Rα and IL-17 A. (A) TriMAP plot of all immune populations in the index patient for all time points before and after treatment initiation. (B) TriMAP plot showing changes in immune cell types before and after treatment, stratified by time point. Differences in immune populations before and after treatment are colored gray. (C) TriMAP plot of the expression of each individual marker in the index patient. (D) Representative histograms show the identification of four immune population clusters with breakdown of markers in each cluster. (E) Bar plots showing percentages of subtypes of granulocytic and monocytic cell populations in the three timepoints before and after treatment; DC, dendritic cells. (F) Illustration showing immunopathogenic populations in the whole blood of the index patient that decreased with treatment. (G) Quantification of mean fluorescence intensity (MFI) and absolute numbers of MRGPRX2-producing cells between different timepoints. (H) Quantification of mean fluorescence intensity (MFI) and absolute numbers of IgE-producing cells between different timepoints. (I) Bar plots showing expression of MRGPRX2 and IgE at the three timepoints. T0, before treatment, T1 and T2, after treatment.
