Fig. 4
From: An adult myogenic cell line of the Japanese fire-bellied newt Cynops pyrrhogaster
Changes in the immunoreactivity of Myf5, MRF4 and myogenin during forearm regeneration. (a) Intact muscle of the forearm. Transverse sections of muscles are shown. Red: Myf5, MRF4 and myogenin. Arrowheads point to the immunoreactive area. Green: myosin heavy chain (MHC). Blue: DAPI nuclear stain. Scale bar, 50 μm. (b) Presumptive myogenic cells in blastema. Sections along the dorsoventral axis are shown. White line indicates the amputation plane. RFP: negative control. Scale bars, 500 μm (left); 100 μm (center); 50 μm (right). (c) Differentiating muscle cells in regenerating forearms at the digit stage. Sections along the dorsoventral axis are shown. Car: cartilage. Scale bars, 50 μm.
