Fig. 6
From: An adult myogenic cell line of the Japanese fire-bellied newt Cynops pyrrhogaster
Characterization of CpM01 by scRNA-seq analysis. (a) Representative images of Pax7 immunoreactivity. A satellite cell marker, Pax7, was detected in migrating cells around a 4-week-old muscle explant (left column; n = 4), but not in CpM01 (right column; n = 2). Blue: DAPI nuclear stain. Scale bars, 200 μm. (b) Comparison among CpM01, erythroid cells and retinal cells by principal component analysis with their scRNA-seq data (see Methods). These cell types were separated into different clusters on the t-SNE map. (c) A heat map visualized the top 20 genes expressed at significantly higher levels in each cell type. (d) Expression patterns of myogenic genes in these clusters. Transcripts of myogenic genes, including Pax7, were detected in CpM01. (e) Five subclusters of CpM01 visualized on the UMAP map. (d) Violin plots showing the expression patterns of cell cycle marker genes and myogenic genes in the subclusters.
