Fig. 2
From: Mouse embryonic kidney transplantation identifies maturation defects in the medulla
Identification of maturation coordinates of the renal medulla in vivo. (a) scRNA-seq analysis of transplanted embryonic kidneys (day 8 and 12) and developing kidneys (E15.5, P0, P7). The day 12 grafts include the sample (*) from our previous report18. The UMAP plots are shown. NP: nephron progenitor; Pod: podocyte; PT: proximal tubule; LoH: loop of Henle; DT: distal tubule; UB: ureteric bud; CD: collecting duct; EC: endothelial cell; Mφ: macrophage; Ren: renin cell; Mes: mesangial cell; SP: stromal progenitor; iMS: inner medullary stroma; oMS: outer medullary stroma. (b) Volcano plots showing the differentially expressed genes between the renal medulla (CD, iMS, oMS) at E15.5 and P7. Red circles: representative genes that are more abundantly expressed at E15.5. Blue circles: representative genes that are more abundantly expressed at P7. (c) CNET plots showing the differentially expressed genes between the renal medulla (CD, iMS, oMS) at E15.5 and P7. Representative genes are shown in circles. (d) Heatmaps of the developing kidneys (E15.5, 17.5, P0, and P7) using the top 200 maturation coordinates. The representative genes are listed on the right side of the heatmaps for each cell type.
