Fig. 5
From: Mouse embryonic kidney transplantation identifies maturation defects in the medulla
Administration of the HIF1 activator does not ameliorate medullary maturation defects. The treated and untreated grafts transplanted into the peri-testicular fat were compared histologically at day 12 after transplantation of E12.5 kidneys. Two independent transplantation experiments were performed (two grafts in each condition per experiment). (a) H&E staining of the grafts with and without Roxadustat treatment. Scale bars: 500 μm. (b) In situ hybridization of the CD maturation markers (Aqp4 and Aqp2) in the grafts with and without Roxadustat treatment. Scale bars: 500 μm. 2nd row: magnified images of the 1st row. Scale bars: 100 μm. (c) In situ hybridization of the iMS maturation markers (Hif1a and Ptgs2) in the grafts with and without Roxadustat treatment. Scale bars: 500 μm. 2nd row: magnified images of the 1st row. Scale bars: 100 μm. (d) In situ hybridization of the neuronal genes (Cntn6 and Gria1) in the grafts with and without Roxadustat treatment. Scale bars: 500 μm. 2nd row: magnified images of the 1st row. Scale bars: 100 μm.
