Fig. 6
Curcumin promoted dendritic growth of primary hippocampal neurons subjected to hypoxia stress in vitro. A, B Effects of curcumin on cell viability and cytotoxicity of primary neurons subjected to hypoxia stress. C Representative images of MAP2 staining. D, E Quantification of total cultured neuron numbers and total dendritic length. F, G Western blot of PSD95 and BDNF in the hippocampus neurons (the original blots/gels are presented in Supplementary Fig. 2). The histogram below shows the relative protein band density. Con = control group; H4h = neurons treated with 0-0.1% O2 for 4 h group; cur-1/2/3/4/5 = curcumin (1, 5, 10, 20, 25 µM) groups; H14d + Cur = H14d + curcumin group. All data are expressed as mean ± SEM, n = 3, *p < 0.05, **p < 0.01 versus Con group, #p < 0.05, ##p < 0.01 versus H4h group.
