Fig. 5
Mutation of EXO70A1 resulted in increased density of cortical CESA compartments. (A) Representative single frames taken at cortical (−0.3 μm below PM) and subcortical (−0.6 μm below PM) focal planes in hypocotyl epidermal cells of WT and exo70a1-1. The epidermal cells of 3-day-old etiolated hypocotyls were observed. Scale bar = 5 μm. (B) Quantitative analysis shows that the number of CESA compartments was increased significantly in the cortical but not in the subcortical cytoplasm in exo70a1-1. n = 29 cells in WT, n = 27 cells in exo70a1-1, a total of 1482, 2019, 805, and 873 compartments were captured from total areas of 44,656, 39,623, 46,399, and 40,047 μm2 in WT cortex, exo70a1-1 cortex, WT subcortex, and exo70a1-1 subcortex, respectively. Error bars represent SD. ANOVA was performed, letters [A-C] denote groups that show statistically significant differences with other groups, p < 0.01. Images of (A) were captured with SDCM.
